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Plant Tandem CCCH Zinc Finger Proteins Interact with ABA, Drought, and Stress Response Regulators in Processing-Bodies and Stress Granules. ... Resulting positive single colony was inoculated into 5 ml of LB and grown overnight at 28°C until OD 600 reached 0.8.

The product mixture (0.424 μmol/mL) was then allowed to react with trypsin (5.97×10 −4 μmol/mL) and carboxypeptidase B (1.93×10 −4 μmol/mL). After 30 minutes, the reaction was quenched by the addition of 1.58 mL of 1% trifluoroacetic acid in acetonitrile.

Abscisic acid (ABA) was functionalized at the 3′-position by reaction of its α-2′,3′-epoxide with 1,4-butanedithiol to afford the corresponding derivative having a six-atom linker arm containing a terminal aulphhydryl group.

For pedicle feeding of ABA, individual clipped flowers were placed inside 1.7 mL microcentrifuge tubes (with their caps removed) containing 100 μL of either water or ABA (1 μg mL-1) dissolved in water. The microcentrifuge tubes carrying the flowers were placed inside …

Abstract. The phytohormone abscisic acid (ABA) regulates essential growth and developmental processes in plants. Recently, RNA-binding proteins have been described as components of ABA signaling during germination.

During seed development, embryonic ABA is required to impose lasting dormancy (Nambara and Marion-Poll, 2005), and de novo ABA synthesis in the embryo during imbibition ensures maintenance of dormancy (Kucera et al., 2005).

Abscisic acid (ABA) is a major phytohormone involved in important stress-related and developmental plant processes. Membrane-delimited ABA signal transduction plays an important role in early ABA signaling, but the molecular mechanisms connecting core signaling components to the plasma membrane remain unclear.

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) Inventor Nnochiri N. Ekwuribe Christopher H. Price James Gordon Still Jennifer Ann Filbey Aslam M. Ansari Amy L. Odenbaugh Balasingam Radhakrishnan Current Assignee (The listed

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External ABA (Na + ABA) increased Na + uptake by 9% and especially favored Na + translocation to the stems (38%) and the leaves, and these latter organs had a 77% higher translocation than the NaCl-treated plants (Na).

ABA quantification The ABA contents in different bush bean plant tissues treated with 1 or 25 mM NaCl, determined by the different methods tested, are given in Table I. Extracts from the 25 mM NaCl stressed plants contained up to twice the ABA concentration present in the non-stressed 1.0 mM NaCl controls.

For ABA or H 2 O 2 treatment, 10 μM ABA or 1 mM H 2 O 2 was added directly into the medium of the suspension cells. Membrane potential values were collected 1 min after addition of the effectors. Membrane potential values were collected 1 min after addition of the effectors.

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In Arabidopsis thaliana, SALT OVERLY SENSITIVE 2 (SOS2) and SOS3, its protein-interacting partner, have been described as central players in salt-stress responses, while another SOS2-interacting partner, ABSCISIC ACID (ABA)–INSENSITIVE 2 (ABI2), is involved in ABA signaling.

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The recovery rates of ABA and GA 3 were calculated based on the 50 ng ml − 1 and 100 ng ml − 1 standards added. The mean rates of recovery of ABA were 96.6% and 97.5%, respectively, and of GA 3 were 95.2% and 96.3%, respectively.

Extraction of endogenous guard cell ABA from epidermal strips was done by adding 1 ml extraction solution (5 ng of [2 H 6](+)‐cis, trans ABA internal standard (Olomouc, Czech Republic) in methanol‐water, 7 : 3) to 10 mg of dried and ground material.

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Purified proteasome (0.15 mg/ml) was incubated with 1 μg/ml of PARP and 10 μg/ml of DNA (sonicated 10 times for 20 s) in a medium consisting of 100 mM Tris, 200 μM NAD +, …

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Abstract. The CXC chemokine interleukin-8 (IL-8/CXCL8) induces rapid mobilization of hematopoietic progenitor cells (HPCs). Previously we showed that mobilization could be prevented completely in mice by pretreatment with neutralizing antibodies against the β2-integrin LFA-1 (CD11a).

1) Ajouter 1 mL de solution d’extraction et finaliser le broyage. 2) Ajouter 1 mL de la solution d’étalon interne 1 (α-ABA). 3) Transvaser dans un tube eppendorf de 2ml

May 22, 2015· Abscisic acid (ABA) is a plant hormone involved in the response to environmental stress. Recently, ABA has been shown to be present and active also in mammals, where it stimulates the functional activity of innate immune cells, of mesenchymal and hemopoietic stem cells, and insulin-releasing pancreatic β-cells.

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The ABA stock solution was prepared by pre‐dissolving 2 mg of (+/–)ABA powder (Sigma A7362) in 0.5 ml absolute ethanol and then finally making up to 1 ml with water. After dilution to 0.37 µM ABA the final ethanol concentration was only 0.005% (v/v).

Transcriptome profiling identifies ABA mediated regulatory changes towards storage filling in developing seeds of castor bean (Ricinus communis L.)

We also wish to thank Philippe Barrieu (Ecophysiologie des Plantes sous Stress Environnementaux, Institut National de la Recherche Agronomique, Montpellier, France) for endogenous ABA measurements and Hugues Baudot for help with raising the plants.

Promoters of ABA-responsive genes have specific ABA cis-acting elements able to bind trans-acting factors (Finkelstein et al., 2002). By contrast, knowledge of the sequence of events linking ABA perception to nuclear events is more fragmentary, although an ABA receptor was recently identified ( Razem et al., 2006 ).